Journal: The Journal of Immunology Author Choice

Article Title: Repositioning the Early Pathology of Type 1 Diabetes to the Extraislet Vasculature

doi: 10.4049/jimmunol.2300769

Figure Lengend Snippet: Anatomy of lymphatics in normal and diseased human pancreatic islets. (A) No LYVE-1 islet-associated staining was detected in tissue from normal donors. (B and C) Lymphatics were present in islets from type 1 diabetes donors. (D) Similar to the NOD mouse lesion, CD4 T cells accumulated on the outside of the islet and were associated to lymphatic vessels. (E) Based on the intensity of the insulin stain, the successive steps of the human lesion are shown in a single specimen in which, from left to right, normal islets, lymphatic-associated islets, and nearly and fully destroyed islets were found. Zoomed-in regions where LYVE-1 staining was present showed the intimate relationship between lymphatics and the outside of the islet. Scale bars, 50 µm.

Article Snippet: Normal human and diabetic pancreatic tissues were obtained from the Network for Pancreatic Organ Donors with Diabetes (nPOD) program and MyBiosource.

Techniques: Staining

Journal: Communications Medicine

Article Title: Host factor PLAC8 is required for pancreas infection by SARS-CoV-2

doi: 10.1038/s43856-025-00745-6

Figure Lengend Snippet: a Viral infiltration occurs in both endocrine (Islets) and exocrine pancreas (Ducts and Acini). Sections are stained with anti- SARS-CoV-2 Spike Glycoprotein S1 (Spike, red) and counterstained with Hematoxylin. Bar, 100 µm; b Plasma levels of pancreatic injury biomarker pancreatic lipase stratify COVID-19 patients according to severity; c Patients (%) with pancreatic lipase above normal blood levels (>70 U/L); d Plasmatic levels of pancreatic lipase in hospitalized patients that ultimately were discharged or who died; e Plasma levels of pancreatic injury biomarker pancreatic amylase stratify COVID-19 patients according to severity ; f Patients (%) with circulating pancreatic amylase above normal blood levels (>90 U/L); g Plasmatic levels of pancreatic amylase in hospitalized patients that ultimately were discharged or who died. N = 40 healthy donors; 40 severe patients; 40 critical patients. Data are presented as mean ± s.e.m. Statistical significance was calculated by ordinary two-tailed unmatched t-test.

Article Snippet: Normal pancreatic tissues were obtained from Zyagen.

Techniques: Staining, Biomarker Assay, Two Tailed Test

Journal: Communications Medicine

Article Title: Host factor PLAC8 is required for pancreas infection by SARS-CoV-2

doi: 10.1038/s43856-025-00745-6

Figure Lengend Snippet: a PLAC8 expression pattern in pancreatic sections of non-infected (Control; n = 3) and infected (COVID-19; n = 3) deceased patients. Sections are stained for PLAC8 protein (red) and counterstained with Hematoxylin (blue). Rectangles mark areas of higher magnification in the bottom row. Bar, 100 µm; b Pancreatic Islets and Acini were manually identified in necropsies and blinded quantified using QuPath. Data are presented as mean ± s.e.m. of PLAC8 positive area relative to the total area for uninfected donors (Control; n = 2), and infected patients (COVID-19; n = 3). Statistical significance was calculated by ordinary two-tailed unmatched t-test.

Article Snippet: Normal pancreatic tissues were obtained from Zyagen.

Techniques: Expressing, Infection, Control, Staining, Two Tailed Test

Journal: Communications Medicine

Article Title: Host factor PLAC8 is required for pancreas infection by SARS-CoV-2

doi: 10.1038/s43856-025-00745-6

Figure Lengend Snippet: Morphometric analysis of a PLAC8/SARS-CoV-2-N staining of pancreatic tissue from non-infected controls ( a ) and COVID-19 patients ( b ). The fluorescence intensities in the indicated fluorescence channels along the white line were measured. Scale bar, 100 µm.

Article Snippet: Normal pancreatic tissues were obtained from Zyagen.

Techniques: Staining, Infection, Fluorescence

Journal: Oncotarget

Article Title: IL2RG, identified as overexpressed by RNA-seq profiling of pancreatic intraepithelial neoplasia, mediates pancreatic cancer growth

doi: 10.18632/oncotarget.19848

Figure Lengend Snippet: Heirarchical heatmap clustering of similarity to tissue-based profiles of alternative splicing. Isoform expression in PanINs and normal ducts was compared to a curated database of isoform expression profiles in different tissues. The 12 profiles included in the heatmap are those that most strongly correlated with the PanIN and normal pancreatic duct samples. Normal pancreatic duct samples correlated strongly and almost exclusively with the normal pancreas profile. PanIN-2 and PanIN-3 samples were less related to the normal pancreas profile than PanIN-1 samples and more similar to other tissues (colon, fetal large intestine, and fetal small intestine).

Article Snippet: Tissue microarrays (TMAs) of pancreatic ductal adenocarcinoma, PanIN and normal pancreas tissue from patients who had their pancreatic cancer resected at Johns Hopkins Hospital (Baltimore, MD) were analyzed as previously described [ ].

Techniques: Alternative Splicing, Expressing

Journal: Oncotarget

Article Title: IL2RG, identified as overexpressed by RNA-seq profiling of pancreatic intraepithelial neoplasia, mediates pancreatic cancer growth

doi: 10.18632/oncotarget.19848

Figure Lengend Snippet: A. Expression of IL2RG transcript in normal pancreatic duct and PanIN. Values are in Log 2 transcripts per million (TPM). B. IL2RG and lymphocyte markers PTPRC and CD3D transcript levels in normal pancreatic duct and PanIN samples. C. Immunohistochemical analysis of IL2Rγ in human normal pancreatic duct, PanIN, and pancreatic cancer tissues, D. Orthotopic implants of mouse pancreatic cancer cell lines.

Article Snippet: Tissue microarrays (TMAs) of pancreatic ductal adenocarcinoma, PanIN and normal pancreas tissue from patients who had their pancreatic cancer resected at Johns Hopkins Hospital (Baltimore, MD) were analyzed as previously described [ ].

Techniques: Expressing, Immunohistochemical staining